![]() With western blot bands, the "base" is the area where the band meets the membrane background. Mountain height can be thought of in terms of their height above sea level, but if you want to climb a mountain, you are really interested in how tall the mountain is from its base. Image analysis software typically offer tools to subtract background, depending on the method of band quantification used.Īn intuitive way to think about background is to think about how we measure the size of mountains. For situations where the background intensity is near that of the signal, or where the background is uneven, identifying which pixels belong to the band of interest, and identifying the relevant background to subtract can be challenging. This background can come from non-specific binding during antibody incubation, membrane background in the case of fluorescent images, or background arising from the instrumentation itself.įor bright, isolated bands on smooth backgrounds, this process can be relatively straightforward. No western blot is perfect, and all blot images show some level of background. Therefore, band quantity is often referred to as band "volume" by imaging software. Each pixel also carries signal intensity information, which can be thought of as data in the third dimension. ![]() The pixels that comprise the part of a band have x and y coordinates, so outline an area. Therefore, band quantity can be thought of as a volume, which is the total amount of signal for all the pixels within that band. Each pixel in a blot image has an x and y coordinate, in addition to an intensity value, which occupies the third dimension. A digital image of a blot can be thought of as data in three dimensions. A western blot image is made up of pixels, which contain information about how much signal was collected at each location in the image. Band VolumeĪ protein band is a feature that appears in a western blot image. Note: western blots are semi-quantitative so they are ideal for showing relative protein expression rather than an absolute quantity. By analyzing the intensity of the signal, you may determine whether the expression of the target protein in one sample has increased or decreased relative to another sample or control. The signal intensity of the band is directly proportional to the concentration of your target protein. Quantitating a western blot refers to the measurement of the signal emitted by your protein band(s) of interest. Blot Quantitation and Background What Does Quantitation Mean?
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![]() Similar details are recorded for all the other parishes around the Estuary and along the south coast of Devon. In the parish of Malborough in 1619, which then included Salcombe, lived 104 mariners, 5 shipwrights and 2 “coopers barrel makers for sea”. His Lord High Admiral, the Duke of Buckingham, demanded of his county subordinates comprehensive parish lists of all those men with maritime skills. The ambitions of the young King Charles I required the rebuilding of a navy much run down after its Elizabethan successes. Most history until recently has been that of great men and their deeds but the maritime surveys of Devon of the early 1600s tell us a very different story. Unusually, we know something of the common people of the town a few years later. The war with Spain dragged on until 1603 but appears not to have affected Salcombe again. She came ashore on Bolt Tail near Hope Cove on 6 November with 158 survivors, all of whose names are known. The only Spanish ship to be wrecked in England, as distinct from Scotland and Ireland, was the hulk San Pedro Mayor, which served as a hospital ship. It has been stated, but not confirmed, that when the Armada finally appeared in local waters on 31 July 1588 the villages round the Salcombe Estuary had fitted out 16 small ships to support the English fleet. Two years later another survey shows that 5 ships under 60 tons belong to Salcombe with an aggregate tonnage of 150. In July 1570 a census of “mariners mustered in Devon” was taken – 56 are listed for Salcombe and 12 for Portlemouth. John Leland described the harbour and settlement in the 1530s in his Travels in Tudor England as “…sumwat barrid and having a Rok at the entering into it … and aboute half a Mile within the Mouth of the Haven … is Saultcombe, a Fisshar Towne”.Īs relations between England and Spain deteriorated in the 1550s, culminating in the ‘Spanish Armada’ campaign of 1588, new records of town and harbour become available. ![]() The town had been awarded a grant in 1377 “in aid of fortification” but apparently nothing had been done. In 1403 Salcombe was raided by a force from France which had previously sacked and burnt Plymouth. Twelve “barges” and a “ballinger” were hired to transport troops to Brittany at the start of the Hundred Years War. ![]() However, ships of some size were already based in the harbour, referred to by 1342 as ‘Portlemouth’. Maybe it was little more than a fishing hamlet with a few ‘cellars’, simple buildings where farmers living a mile or two inland kept their nets and gear. References to Salcombe are limited for several centuries after 1244, perhaps because of a lack of literate inhabitants (the illiterate leave no records). Archaeologists have identified stone age settlements on the cliff tops on both sides of the mouth of the estuary and a recently discovered shipwreck has demonstrated the existence of cross channel trade some 3,500 years ago. While Salcombe itself may be a latecomer, man has lived around the area from time immemorial. We know about these because their names and assets figure in the Domesday book, William’s comprehensive survey of his new kingdom published in 1083. The manor was the smallest of the administrative units into which England was divided by William I after he conquered the country in 1066. It fell within the boundaries of the parish of Malborough on the edges of two ‘manors’, Batson and West Portlemouth. The name Salcombe first appears in writing in 1244, centuries after most of the other neighbouring settlements were identified. Some were eventually ransomed but others never returned home. Hundreds of Devon people were kidnapped in the 1600s and taken to the slave markets of Algiers and Sallee on the Atlantic coast of Morocco. Others include pirates seeking temporary shelter and supplies and, in the case of those from the Barbary States of North Africa, slaves. Some of these dangers are mentioned in the following paragraphs. Long after the invasions which some of us learned about at school – Roman, Anglo-Saxon, Viking and Norman – it still remained a source of danger. The reason was that danger came from the sea. The oldest local settlements were not built at the water’s edge but at some distance inland. Fishing, seafaring, boat and later shipbuilding with smuggling and probably some piracy were the principal occupations. Until about 100 years ago Salcombe earned its living from the estuary and the sea. Photos Reproduced courtesy of Francis Frith. ![]() Record virtual instruments, drum machines, guitars or audio from the real world. Advanced warping lets you change the tempo and timing of any audio in real time without stopping the music. Use its timeline-based workflow or improvise without constraints in Live’s Session View. Live also comes with a series of built-in interactive lessons, which are available from Live → Help Menu → Built In Lessons.Live is fast, fluid software for music creation and performance. To learn more about using Live, select a topic from our Learn Live tutorials or access the Live Manual from Live → Help Menu → Read the Live Manual. Once Suite is installed you will be able to access Max for Live within Live's Browser. Max for Live is built into Live 10 and 11 Suite. You can also buy additional Live Packs in Ableton's online Pack store. Once Live is installed, you can install these Live Packs, which will add additional sounds, presets and other content in a dedicated directory on your hard drive. Several Live Packs are available for download from the Packs section of your account. If you'd prefer to authorize offline, step-by-step instructions are available. ![]() When starting Live for the first time, follow the instructions on the authorization dialog in order to authorize Live online. Once the installation has completed, click on the Windows Start menu where you'll find the Live 10 shortcut. Make sure that Live is installed to the default location: C:\ProgramData\Ableton\ The setup wizard will guide you through the steps necessary to install Live 10 on your Windows machine. ![]() Live 10:Ĭhoose "Extract All." from the drop down menu and launch the Setup.msi file by double clicking it once the file has been unpacked. Note: The two Data.cab files extracted with the Installer.exe do not need to be decompressed further. Make sure that Live is installed to the default location: C:\ProgramData\Ableton\ The setup wizard will guide you through the steps necessary to install Live on your Windows machine. Right-click on the downloaded Zip file to unpack it.Ĭhoose "Extract All." from the drop down menu and launch the "Ableton Live 11 Installer.exe" file by double clicking it once the file has been unpacked: These may be lost when you uninstall or (auto-)update Live. Note: Do not save or copy any content, files, samples or projects into Live's installation folder. You can also add it to your Dock for quick access. You can then open the Applications folder in Finder and double click on the Live application. Once it is finished, close the window and eject the. dmg and the installer will open automatically.ĭrag the Live icon onto the Applications folder shortcut. When in doubt, please check the latest Apple Silicon and macOS compatibility information provided by each plug-in developer. However, this isn't necessary if your plug-ins are updated for Apple Silicon. Note: Y ou can force Live to run as an Intel application via Rosetta 2 on an Apple Silicon-based Mac, as explained here.
![]() They’re ex-big label, but now independent. The Counting Crows are obviously a big name as well, and we wrote about their decision to use BitTorrent. In case you’re wondering who’s actually offering up music that’s getting downloaded like this, Eliot van Buskirk tracked down the top ten authorized music acts on BitTorrent, which turns up a few surprises.Īs Eliot notes, that number one legal download, Death Grips, is signed to a major label deal on Epic (part of Sony Music). And that’s because it’s showing 689,955 authorized downloads. If you go to the full page, there’s an awful lot of scrolling involved. ![]() We’ve put a snapshot of just a small portion of that image below this post… but that’s really only a fragment. put together a neat graphic representation of just one day’s authorized downloads, creating a massive page that includes a single dot for every authorized download. In an effort to highlight just how much authorized content is shared using BitTorrent, Bittorrent Inc. It seems quite likely that the trend is moving in the right direction. Now, you can argue that this is still less than half of all files - but it’s a big step up from the standard claims that somewhere between 1% and 10% were authorized. The recent MusicMetric analysis of BitTorrent downloads for the first six months of 2012 found that 31% of downloads were for authorized files. And that seems to be happening with BitTorrent. Content creators begin to embrace the new, realize that it might not be evil, and suddenly we see more and more interesting case studies. The argument being made is that there is little redeeming value with BitTorrent since it’s almost exclusively used for infringement. Lately, there has been plenty of talk about BitTorrent - with a few cases here and there pointing out that a high percentage (usually over 90% of works are infringing). However, people don’t often deal with trends very well, and they assume, quite incorrectly, that if a technology is initially used in an unauthorized manner, it must be a “piracy tool” and no amount of discussing how trends and adaptation works will convince them otherwise. Early on, all sorts of new and innovative technologies are mostly used for unauthorized copies… until the industry catches up. Of course, thankfully, the Supreme Court eventually made it clear that just because it was “unauthorized,” it didn’t mean that taping TV for later watching was “infringing.” But, if the metric you used to judge whether or not a new technology is a “pirate technology” is what percentage of its use was “unauthorized,” you get a very skewed picture. When the VCR first came on the market, nearly 100% of the TV and movie content it was used for was “unauthorized,” because the big studios refused to offer films. ![]() ![]() ![]() “We are following up robustly with all pension schemes administered by Capita to ensure they do so.” “We are calling on all trustees to work with Capita to understand how their scheme may have been impacted, to fulfil their responsibilities as data controllers and to warn members of the threat of scams and how to protect themselves,” said the regulator. The software targeted by the cyber criminals is called Hartlink, which pension schemes can install on their websites to create a secure link for pensioners to view and manage their investments.Ī spokesman for the Pensions Regulator described the fallout from the March cyber attack as “an ongoing situation with more detail emerging daily”. “We are very confident members’ pensions remain secure”.įor weeks after the attack first came to light in April, Capita insisted that there was no evidence any data had been accessed by hackers, even as stolen passport scans and other financial information was shared on a dark web site run by the Russian Black Basta criminal gang. We have given them guidance on the risks this might have created and how they might respond.” Mr Galvin added: “Having been told that Capita could not guarantee the security of certain files, we’ve moved urgently to inform our members. Data privacy and security is a top priority for us, and we know our members will be concerned by this news.”ĭata stolen from the USS included pensioners’ names, dates of birth, and National Insurance numbers among other details. USS said it would write to all affected “active, deferred and retired” members even as regulators scramble to understand the full impact of the hack.īill Galvin, USS chief executive, said: “We are very sorry that some USS member data held by Capita may have been accessed by a third party. It is thought similar information was accessed at the other funds. Industry sources said the March cyber attack affected up to 350 corporate retirement schemes across the UK, making it the largest such hack in British history.įunds affected included the Universities Superannuation Scheme (USS), the biggest private pension pot in Britain, which said on Friday that 470,000 of its members had had their data accessed by criminals through a piece of Capita software it uses.ĭata stolen from the USS included pensioners’ names, dates of birth, and National Insurance numbers among other details. Personal data belonging to millions of retirement savers were targeted by hackers after more than 300 pension funds were hit by the Russian-linked cyber attack on IT outsourcer Capita. ![]() ![]() Furthermore, Wenkel and Naumann 13 reported the retrolaminar changes in the optic nerve after SO instillation with secondary angle-closure glaucoma. 2c).Īlazemi and colleagues 12 disclosed the direct migration of liquid SO from the breast to the mediastinum. SCI (Oculus) showed heterogeneous, highly reflective, tiny scattered areas in the upper half of the intrastromal space in OD ( Fig. On slit-lamp examination, haze on the upper half of the corneal stroma and intrastromal tiny, bright, and emulsified SO particles were noted. Intraocular pressure was 8 mmHg, vision remained hand movement. Six months later, mild corneal haziness developed. Slit-lamp examination findings on the first postoperative day and month were normal. Repeat vitrectomy, SO extraction, preretinal membrane peeling, internal drainage of subretinal fluid, endophotocoagulation, and intravitreal gas (C3F8) tamponade were performed. Five months later, retinal redetachment developed in the eye. At that time, the cornea was not injured and the retina was attached. 7 The SO bubble was removed spontaneously with this corridor.Ī 34-year-old man underwent 23-gauge vitrectomy, intraocular foreign body removal, and 1000-centistoke SO tamponade combined with phacoemulsification on his right eye due to scleral penetrating injury with a piece of metal wire. Additionally, air was then injected with a 30-gauge cannula between the deep stroma and Descemet membrane to separate those layers, as described previously. Intravitreal SO was removed via pars plana sclerotomies. SCI (Oculus) revealed a sharply demarcated, wide, hyporeflective area between the corneal stroma and Descemet membrane in the right eye ( Fig. The diameter of the bubble was about 6 mm. Additionally, an SO bubble was detected in the center of cornea ( Fig. Right eye was aphakic, and the anterior chamber was completely filled with SO. Slit-lamp examination revealed circumciliary congestion, diffuse stromal corneal edema, and bullous keratopathy in the right eye. Translucent cornea, fibrous pupillary membrane, and posterior synechiae were detected. The sclera was thickened, and the intraocular contents were atrophic and disorganized. Slit-lamp examination showed shrinkage in left eye. Intraocular pressures were 14 and 5 mmHg applanation in the right and left eyes, respectively. His best-corrected visual acuity was hand movement OD and no light perception OS. Pars plana vitrectomy and 1000-centistoke SO tamponade had been performed. The last surgery (11 months prior to his presentation to the hospital), performed because of recurrent retinal detachment, was performed in a different hospital. His right eye past ocular history included 3 retinal detachment repair procedures between 20. Subsequently, he developed phthisis bulbi in the left eye. Relevant past ocular history included 4 retinal detachment repair procedures on the left eye between 19. A 56-year-old man was admitted to the hospital for decreased visual acuity in the right eye. When carrying out your research for a piece of work, you will need to search more than one database to find all of the journal. These databases have been especially chosen for this subject area. this volume features contributions that: provide a conceptual clarification of many different terms that are used for describing deprived communities and offer a systematic literature review on community processes and effects on well-being in underdeveloped communities map different fields of social work involvement in deprived communities with concrete practice examples and, stress why social work as a profession needs support and how it can be empowered to improve its capacities in deprived communities. Databases provide access to high-quality peer-reviewed journal articles, conference proceedings, book chapters, dissertations and other sources. Deprived communities, used in this book to mean slums, ghettos, favelas, and low-income, remote, underserved, vulnerable, impoverished, underdeveloped, disadvantaged, or less-favoured communities, exist worldwide and are conceptualized under different terms and concepts. This contributed volume offers a holistic understanding of social work practice in deprived communities through its thematization of understanding deprived communities globally, the development of competencies for social work practice in and with deprived communities, social work education as a community development tool, and the empowerment of social workers in deprived communities. Social Work is dedicated to improving practice and advancing knowledge in social work and social welfare. Widely read by practitioners, faculty, and students, it is the official journal of NASW and is provided to all members as a membership benefit. The database provides in-depth and non-evaluative abstracts of journal articles and citations to book reviews drawn from over 1,800 serial publications, and also provides abstracts of books, book chapters, dissertations, and conference papers. Contributors to the volume are from different backgrounds and trainings, and write on such topics as: the vicious cycle of white centrality being Black in a world of whiteness undoing internalized white supremacy intersectionality and the contradictions of a white, Jewish identity becoming an antiracist leader and building an antiracist clinical practice. Social Work is the premiere journal of the social work profession. ![]() Being intentional about naming, deconstructing, and dismantling whiteness is a precursor to responding effectively to the racial reckoning of our society and improving race relationships, addressing systemic bias, and moving toward the creation of a more racially just world. An up-front, close, and fresh examination of the impact of whiteness and how it contributes to our troubled race relationships, this book posits that whiteness is a pervasive ideology that is rarely overtly identified or examined, although it has profound effects on race relationships in therapy and beyond. ![]() A comprehensive collection on the topic of whiteness from writers in the field of mental health and activism. ![]() □ For everyone who can’t install Adobe Products on Mac and to overcome applications errors you should first Clean your Mac Before you Install it. □ Clean first your mac before installing Adobe Products ▶️ Open the link by using Safari or Chrome : To avoid errors like system compatibility report or error code 195 or quit unexpectedly mac applications, INCOMPATIBLE M1 or M2, If you have Chip M1 or M2 use the following instructions to Install Adobe Products on Chip M1 or M2 and more follow those instructions.Īttention □ ! 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New sliders in the Essential Sound panel let you dial down or remove background noise and reverb for incredible results. Each curve has two axes with paired values, making selective color grading easy and accurate. Take the guesswork out of curve adjustments with innovative Lumetri Color tools. And Premiere Pro integrates with hundreds of partner technologies. Open a Motion Graphics template from After Effects or download one from Adobe Stock and customize it - all without leaving the app. ![]() Right where you need it.Īlong with tools for color, audio, and graphics, Premiere Pro works seamlessly with other apps and services, including After Effects, Adobe Audition, and Adobe Stock. Delivering content that’s optimized for any screen or platform is faster than ever.Įverything you need. Broad native file support and simple proxy workflows make it easy to work with your media, even on mobile workstations. ![]() And automated tools powered by Adobe Sensei speed up time-consuming tasks, so you can focus on your story.Įdit footage in any modern format, from 8K to virtual reality. Integrated, efficient workflows let you polish your work without leaving your timeline. Premiere Pro is the industry leader for editing everything from fast-turn TV series to feature films. ![]() Premiere Pro is used by Hollywood filmmakers, TV editors, YouTubers, videographers - anyone with a story to tell, including you. And Premiere Rush, our new app, is included with your subscription so you can capture footage and start editing on all your devices, anywhere. Creative tools, integration with other Adobe apps and services, and the power of Adobe Sensei help you craft footage into polished films and videos in one seamless workflow. Premiere Pro is the leading video editing software for film, TV, and the web. Title: Adobe Premiere Pro 2024 24.0.3.2 Win/Mac 圆4 Adrenaline, in particular, is the reason your cheeks feel flushed, your palms feel sweaty and your heart races when you meet someone you like for the first time. The emotion of euphoria, and the release of a jumble of chemicals in the brain, including dopamine (pleasure), adrenaline (fight or flight) and norepinephrine (alertness), can make falling in love feel like an addictive rush. Stage 2: Attraction is similar in feeling to the effect of certain drugs or alcohol. ![]() This isn't so different from other mammals on the planet. Stage 1: Lust is driven by the levels of testosterone (men) and oestrogen (women) in our bodies. The 3 stages of loveĪccording to Dr Helen Fisher, a renowned anthropologist, there are 3 distinct phases of falling in love. ![]() The final phase is attachment, which is when you may begin to feel closely bonded and start making long-term plans together. The second, attraction, is similar in feeling to the addictive rush from certain drugs or alcohol. The first, lust, is driven by the levels of testosterone (men) and oestrogen (women) in our bodies. There are 3 distinct phases of falling in love. The initial happy feelings of being in love is stimulated by 3 chemicals in the brain: noradrenaline that stimulates adrenaline production causing that racing heart and sweaty palms dopamine, the feel-good chemical and phenylethylamine that is released when we're near our crush, giving us butterflies in our tummies.īut is there a biological reason behind these feelings? And why do you fall in love with that one person you do? As time passes, this initial bust of feeling often fades into a comfortable closeness. We talk about the moment of falling in love as if we have been hit by Cupid's arrow – it is intense, overwhelming, sometimes fast and can feel like fate. What is the current situation regarding SARS-CoV-2 viral variants? One criterion for a VoC should be that it becomes dominant over other SARS-CoV-2 lineages in circulation. Pi, the next one in line, will probably not look like Omicron or any of its sub-lineages (including XBB1.5). Five VoCs have been identified to date and all have received a Greek letter: Alpha, Beta, Gamma, Delta and Omicron. We use the term to describe the SARS-CoV-2 ‘ Variants of Concern’ (VoCs) flagged by the World Health Organisation (WHO) because those lineages proved to be more transmissible, more virulent, and/or better at evading previous immunity. What we call variant is more restrictive than what most people or the media have in mind. Let’s start by defining the term viral “variant” In this interview, they give us valuable insight on what is driving viral evolution and what to expect in the future. They form part of the EU-funded END-VOC project, which aims to evaluate the circulation and impact of emerging SARS-CoV-2 variants. ![]() François Balloux, director of the Genetics Institute at University College London, and his colleagues Lucy van Dorp and Damien Richard, have been closely watching the virus since the early days of the pandemic. SARS-CoV-2, the virus that causes COVID-19, has shown a remarkable capacity to adapt to its human host over a short period of time. ![]() ![]() Interview on the emergence of new variants of the SARS-CoV-2 virus, which causes COVID-19, with three researchers from the END-VOC project: François Balloux, Director of the Institute of Genetics at University College London, and his colleagues Lucy van Dorp and Damien Richard. AIGHD Senior Fellow, Anniek de Ruijter, is the AIGHD research lead for the END-VOC project, which is explained in detail in the story below. |
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